Muri ADN isanzwe, RNA hamwe na acide nucleique idasanzwe ya acide, intambwe ya Deprotection na Coupling igira uruhare runini.
Intambwe ya Deprotection nugukuraho itsinda rya DMT kumfashanyo ikomeye cyangwa 5 'hydroxyl groupe kuri nucleoside yabanjirije hamwe na acide organic, ikanashyira ahagaragara hydroxyl itsinda ryintambwe ikurikira.Acide ya 3% ya trichloroacetic muri dichloromethane cyangwa toluene ikoreshwa cyane mugukora intambwe yo guta agaciro.Ubwinshi bwa acide trichloroacetic hamwe nigihe cyo guta igihe (igihe cyo guhagarika) byiganje mubuziranenge bwibicuruzwa byanyuma.Kwibanda gake hamwe nigihe cyo guhagarika bidahagije bisiga itsinda rya DMT ridakorewe, bigabanya umusaruro kandi byongera umwanda utifuzwa.Igihe kirekire cyo guhagarika igihe gishobora kuyobora depurine yikurikiranya, ikora umwanda utunguranye.
Intambwe ya Coupling yunvikana kumazi arimo ibishishwa hamwe nubushuhe bwikirere.Ubwinshi bwamazi muri synthesis bigomba kuba munsi ya 40 ppm, biruta munsi ya 25 ppm.Kugirango ugumane imiterere ya anhydrous, synthesis ya nucleic acide igomba gukorwa mubushuhe buke, bityo turasaba abakiriya bacu gukoreshaAmidite Ibikoresho Byasheshwe, irashobora gushonga ifu cyangwa amavuta ya Phosphoramidite muri anhydrous acetonitrile kugirango wirinde guhura numwuka.
Kubera ko gushonga kwa fosifora ni byiza mugihe kitari amazi, hamwe nu mutego wa molekuline wo kwamamaza adsor yamazi muri reagent na amidite, birakenewe guteguraImitego ya molekulari.Turasaba 2 g kugabanura amacupa ya reagent ya 50-250ml, 5g kumacupa ya reagent 250-500ml, 10g kumacupa ya reagent 500-1000ml, na 20g kumacupa ya reagent 1000-2000ml.
Gushonga kwa fosiforamide bigomba gukorwa munsi yikirere cya inert, kandi gusimbuza reagent ya reagent na acetonitrile bigomba kurangira mugihe.Imyitozo ya Capping na Oxidation igomba gukoreshwa vuba bishoboka, reagent zifunguye zitanga ubuzima bubi, hamwe nibikorwa bike mugihe cya synthesis.
Igihe cyo kohereza: Kanama-09-2022