Ihame rya Oligo Synthesizer
Mubyerekeranye na biologiya biologiya nubushakashatsi bwerekeranye na genetique, ubushobozi bwo guhuza ADN bigira uruhare runini.Synthesis ya ADN ikubiyemo gukora ibihimbano bya molekile ya ADN itunganya nucleotide muburyo bwihariye.Kugira ngo ibyo bigerweho, abahanga bashingira ku gikoresho gikomeye kizwi nka synthesizer ya oligonucleotide, kizwi kandi nka ADN synthesizer.
Synthesizer ya oligonucleotide nigikoresho gihanitse gihita gihuza molekile ngufi ya ADN yitwa oligonucleotide.Iyi migozi migufi ya ADN mubusanzwe nucleotide 10 kugeza 100 muburebure kandi nibyingenzi byubaka muburyo butandukanye burimo polymerase reaction reaction (PCR), synthesis ya gene, injeniyeti ya geneti, hamwe na ADN ikurikirana.
Oligonucleotide synthesizers ikora ku ihame rya tekinike izwi nkasynthesis ikomeye.Ubu buryo bwatangijwe bwa mbere n’uwahawe igihembo cyitiriwe Nobel, Dr. Marvin Caruthers mu myaka ya za 70 kandi bunonosowe mu myaka yashize kugira ngo habeho synthèse ya ADN ikurikirana.Oligonucleotide synthesis ikorwa muburyo bwo kongeramo intambwe ibisigisigi bya nucleotide kuri 5'-terminus yumunyururu ukura kugeza igihe ibyifuzo byakusanyirijwe.Buri nyongera ivugwa nka synthesis cycle kandi igizwe na reaction enye:
Intambwe ya 1: De-guhagarika (detritylation) ---------Intambwe ya 2: Guhuza ---------Intambwe ya 3: Gufata -----------Intambwe ya 4: Oxidation
Iyi nzira isubirwamo kuri buri nucleotide kugeza igihe ibyifuzo byabonetse.Kumwanya muremure wa oligonucleotide, uru ruziga rushobora gukenera gusubirwamo inshuro nyinshi kugirango uhuze urutonde rwose. Ubushobozi bwo kugenzura neza buri ntambwe ya synthesis cycle ningirakamaro kuri synthesizer ya oligonucleotide.Reagent ikoreshwa, nka nucleotide na activateur, igomba kuba yujuje ubuziranenge kugirango ikore neza kandi neza.Byongeye kandi, synthesizers isaba kugenzura neza ubushyuhe hamwe nibindi bidukikije kugirango biteze imbere bifuza kandi birinde ingaruka zitifuzwa.
Iyo oligonucleotide imaze guhurizwa hamwe, mubisanzwe iba ikuwe mubufasha bukomeye kandi igasukurwa kugirango ikureho amatsinda asigaye arinda cyangwa umwanda.Oligonucleotide isukuye noneho yiteguye kumurongo wo hasi.
Iterambere mu ikoranabuhanga ryashoboje iterambere ryinshi-ryinjiza oligonucleotide synthesizers ishoboye icyarimwe guhuza amagana cyangwa ibihumbi bya oligonucleotide.Ibi bikoresho bifashisha tekinoroji ya micarray, ifasha abashakashatsi kubyara byihuse amasomero manini ya oligonucleotide kubwubushakashatsi butandukanye.
Muncamake, amahame yinyuma ya oligonucleotide synthesizers azenguruka muburyo bukomeye bwa synthesis synthesis, ikubiyemo intambwe yo kongeramo intambwe ya nucleotide kumfashanyo ihamye.Igenzura ryukuri rya synthesis cycle hamwe na reagent yo mu rwego rwo hejuru ni ngombwa kugirango ikorwe neza kandi neza.Oligo synthesizers igira uruhare runini mubushakashatsi bwa ADN, ifasha abahanga kubyara oligonucleotide yihariye ikoreshwa muburyo butandukanye, bigira uruhare mu iterambere ryibinyabuzima nubushakashatsi bwerekeranye na geneti.
Igihe cyo kohereza: Kanama-01-2023