Sintezike ya ADN na RNA byombi bishingiye ku ngamba zikomeye zo guhuza hamwe na chimiya ya fosifora, fosifora ya ADN ishobora gukoreshwa mu guhuza ibigereranyo bya RNA cyangwa RNA nta yandi mahinduka, kandi reagent muri synthesis ya ADN ishobora gukoreshwa neza muri RNA na acide nucleic acide 'synthesis.Nkuko bigaragara ku gishushanyo cya 1, 2'-hydroxy muri RNA phosphoramidite irinzwe hamwe nitsinda ririnda silil, ni ukuvuga t-butyldimethylsilyl (TBDMS), ikumira ingaruka ziterwa nitsinda rya 2'-hydroxy.Itsinda rinini rya TBDMS ryabujije abantu kwitwara hagati ya fosifora na gride ya 5'-hydroxy ku nkunga ihamye, kandi birasabwa igihe kinini cyo guhuza kugirango tumenye neza guhuza neza.
Kimwe na synthesis ya ADN, synthèse RNA yabanje gukurwa mubufasha bukomeye na aminolysis, hanyuma itsinda rya TBDMS ryaciwe na tetrabutylammonium fluoride (TBAF) cyangwa trimethylamine trihydrofluoride.RNA itemewe ishobora kwezwa no kongera gushyirwaho inzoga na HPLC.
Igicapo 1. Imiterere yimiti yububiko bwibanze muri ADN na synthesis ya RNA.
a) fosifora ya dABz na b) rABz 2'-OTBDMS fosifora.
Iterambere ryibiyobyabwenge bya siRNA bisaba imiterere yimiterere ya RNA kavukire kugirango yongere ibinyabuzima, 2'-hydroxy kuri RNA irashobora gusimburwa nitsinda rya MeO, F na MOE, kandi aside nucleic ifunze (LNA) nayo itanga imikorere myiza mubuvuzi bwa RNA (Ishusho 2).Izi fosifora zitanga ibikorwa bisa nkubwoko bwa ADN ya fosifora yo mu bwoko bwa ADN muri synthesis, kandi uburyo bwo gukora no kweza izo acide nucleique zidasanzwe zirasa na ADN kavukire.
Igishushanyo 2. Imiterere yimiti yubaka mumiti ya siRNA.a) dABz 2-MeO fosifora;b) dABz 2-F fosifora;c) dABz 2-MOE fosifora na d) dABz Ifunze fosifora.
Fosiforani ngombwa muguhuza genes, harimo cyane cyane imiryango ya ADN na RNA nibiyikomokaho, fosifora yose hejuru dushobora gutanga mubipaki.
Turahora tunoza imikorere yibicuruzwa byacu, tunonosora ibikorwa byacu kandi tubone ibisobanuro neza.Ntabwo tuguha gusa ibicuruzwa byujuje ubuziranenge, ahubwo tunaguha amahugurwa na serivisi.Dufite ibikoresho byabakozi babigize umwuga hamwe nitsinda ryiza rya tekinike hamwe nabakozi bashinzwe kubungabunga kugirango duhe abakiriya bacu serivisi nziza nyuma yo kugurisha.
Igihe cyo kohereza: Kanama-09-2022