Ibikoresho byo kweza Oligo yoza

1. Gel Electrophoresis Chromatography Yeza
Koresha denaturing polyacrylamide gel electrophoreis chromatografiya kugirango weze.Umukozi wa denaturing muri rusange ni 4M formamide cyangwa 7M urea, ubunini bwa acrylamide buri hagati ya 5-15%, naho methacrylamide ikaba iri hagati ya 2-10%.
Nyuma ya electrophoresis, hagomba kugenwa umwanya wa acide nucleic acide ya nucleique ikenera kugenwa hifashishijwe imirasire yumucyo ultraviolet, gel irimo aside nucleic acide iracibwa, acide nucleique iravunika kandi ikarekurwa, hanyuma igisubizo cyo kumeneka kikaba cyibanze, kigahinduka, ingano na lyofilize.

2. DMT-On, Isuku rya HPLC
Hitamo uburyo bwa DMT-On mugihe cya synthesis, ibicuruzwa byibanze byashyizwe hamwe kandi byibanda kubushyuhe bwicyumba kugirango ukureho ammonia irenze nyuma ya aminolysis.
Gutandukana byakozwe hakoreshejwe inkingi ya C18 hamwe na acetonitrile na 10% triethylamine-acetike (TEAA) nka eluent.Nyuma yo gukuraho birangiye, iba yibanze, hanyuma itsinda rya DMT rikurwaho na acide trifluoroacetic.Nyuma yo kutabogama, imyunyu imwe na molekile ntoya bivanwa mumiyoboro yaciwe, amaherezo bikamanurwa.

Ubu buryo bushobora kubona ibicuruzwa bifite isuku ihanitse, ariko birakenewe ko twita kubintu bibaho.

3. DMT-Off, Kweza HPLC
Hitamo DMT-Off mugihe cya synthesis, kandi ibicuruzwa bitarimo ibicuruzwa byashyizwe hamwe kandi byibanda kubushyuhe bwicyumba kugirango ukureho ammonia irenze nyuma ya ammonolysis.
Gutandukana byakozwe hakoreshejwe inkingi ya C18 hamwe na acetonitrile na 10% triethylamine-acetike acide mumazi nkibisanzwe.Nyuma yo gutandukana kurangiye no kugereranywa, alikoti ziba lyofilize.

Ubu buryo busaba guhindura neza uburyo bwo gutandukana, kandi burashobora no kubona molekile nziza ugereranije.